Design and validation issues in RNA-seq experiments
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چکیده
منابع مشابه
Design and validation issues in RNA-seq experiments
The next-generation sequencing technologies are being rapidly applied in biological research. Tens of millions of short sequences generated in a single experiment provide us enormous information on genome composition, genetic variants, gene expression levels and protein binding sites depending on the applications. Various methods are being developed for analyzing the data generated by these tec...
متن کاملSupplementary manual . Sequential design of RNA - seq experiments
This manual explains how to use casper to help design RNA-seq experiments, for the main manual please load the package and type vignette(’casper’) at the command prompt. casper provides tools to design RNA-seq isoform expression experiments, both single and multiple sample studies. The former may e.g. estimate relative isoform expression within a gene or perform de novo isoform discovery, where...
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MOTIVATION RNA-seq has been extensively used for transcriptome study. Quality control (QC) is critical to ensure that RNA-seq data are of high quality and suitable for subsequent analyses. However, QC is a time-consuming and complex task, due to the massive size and versatile nature of RNA-seq data. Therefore, a convenient and comprehensive QC tool to assess RNA-seq quality is sorely needed. ...
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High-throughput quantitative DNA sequencing enables the parallel phenotyping of pools of thousands of mutants. However, the appropriate analytical methods and experimental design that maximize the efficiency of these methods while maintaining statistical power are currently unknown. Here, we have used Bar-seq analysis of the Saccharomyces cerevisiae yeast deletion library to systematically test...
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Background: The stress is one of main factors effects on production system. Several factors (both genetic and environmental elements) regulate immune response to stress. Objectives: In order to determine the major immune system regulatory genes underlying stress responses, a learning Bayesian network approach for those regulatory genes was applied to RNA-...
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ژورنال
عنوان ژورنال: Briefings in Bioinformatics
سال: 2011
ISSN: 1467-5463,1477-4054
DOI: 10.1093/bib/bbr004